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KMID : 0377519790040010083
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Chung-Ang Journal of Medicine
1979 Volume.4 No. 1 p.83 ~ p.88
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A Study on the Lipids of Sheep Liverfluke, Fasciola hepatica
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Lee Soon-Hyung
Song Chul-Yong
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Abstract
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The present study was undertaken to observe the quality and quantity of lipids in the adult worms of sheep liverfluke, Fasciola hepatica. Lipid extract was done by the methods of Folch et al. (1957) and Kenny (1952), and the the extracted lipid fractions of the worms were separated by thin layer chromatography. Those fractions were also subjected to perform the quantitative analyses of glycerides, cholesterols and phospholipids. The results obtained were summarized as follows. 1. Total amount of glyceride was 45.29㎎ per gram wet weight of worm tissue: the amount of monoglyceride was 4.46㎎; diglyceride, 13.59㎎; and triglyceride, 25.63㎎ per gm wet weight of worm tissue. 2. Total amount of cholesterol was 8.10㎎ per gm wet weight of worm tissue, and the amount of esterified cholesterol(5.21㎎/gm) was much more than that of free cholesterol (2.15㎎/gm). 3. The following 10 phospholipids were detected in the worm tissue of F. hepatica, i.e., lysophosphatidylcholine, phosphatidylinositol, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylethanolamine and three species of unknown phospholipides.
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